Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction

Detection of bovine herpesvirus 1 (BHV-1) genomic sequences in bovine semen inoculated with BHV-1 by polymerase chain reaction.

Polymerase chain reaction (PCR) technique was applied to detect BHV-1 in bovine semen inoculated with BHV-1. The technique was found to be 10(6) times more sensitive than a non-isotopic dot-blot hybridization method in detecting viral genomic DNA. Of the three primer pairs used, the one chosen from glycoprotein gC appeared to be most sensitive as it could detect up to 0.01 TCID50 of BHV-1 in th...

Detection of Different Iranian Isolates of Bovine Herpes Virus Type-I (BHV-I) Using Polymerase Chain Reaction

Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT

Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 ...

Detection of the ectC Gene in Halomonas Strains by Polymerase Chain Reaction

1, 4, 5, 6-Tetrahydro-2-methyl-4-pyrimidine carboxylic acid (ectoine) is an excellent osmoprotectant. Ectoine and hydroxyl ectoines are of great significance to the biotechnology industry, thus the detection and isolation of ectoine producing bacteria is of great importance. Hence, this study involved the detection of the ectC gene (encoding ectoin synthase enzyme) using polymerase chain reacti...

Evaluation of baculovirus-expressed bovine herpesvirus-1 (BHV-1) glycoproteins for detection and analysis of BHV-1-specific antibody responses.

Baculovirus (Autographa californica nuclear polyhedrosis)-expressed bovine herpesvirus-1 (BHV-1) glycoproteins B (gB), gC, and gD were developed and characterized. The recombinant proteins retained their antigenic properties as determined by immunoblotting against monoclonal antibodies. The proteins were examined as antigens for detection of BHV-1 infection and for the analysis of antibody resp...